Journal: Poultry Science
Article Title: Single-cell RNA sequencing reveals intrahepatic signature related to pathobiology of duck hepatitis A virus type 3 (DHAV-3) infection
doi: 10.1016/j.psj.2025.104798
Figure Lengend Snippet: Dynamic response of T/NK cells during DHAV-3 infection. (A) UMAP plot of 15 subclusters of T/NK population. (B) The ratio of each T/NK subcluster in each group. (C) The boxplot representing ratio of each subcluster in R24 and S24. (D) RNA velocity analysis showing the transition potential among CD8+ T-cell subsets. (E) The correlation between viral load and PLAC8 expression level in viral RNA positive cells. (F) The qPCR results showing the PLAC8 expression and DHAV-3 viral load in multiple tissues from R24 and S24 ducklings. (G) PLAC8 overexpression promoted DHAV-3 replication in primary liver cells. The p values shown in the figures are represented by *p<0.05, **p<0.01, ***p<0.001.
Article Snippet: Recombinant plasmids pcDNA3.1- PLAC8 and pcDNA3.1- ANGPTL4 were constructed by double digestion and verified by Sanger sequencing (Sangon Biotech, China).
Techniques: Infection, Expressing, Over Expression